Flow backward alleviated the river algal blooms.

Mechanistic understanding and prediction of river algal blooms remain challenging. It is generally believed that these blooms are formed by the slowdown of water dynamics in tributaries due to the support of the main stream. However, few studies have investigated the impact of flow backward caused by the difference in water dynamics between the main stream and tributaries. Here, we focus on the eutrophication issue in the middle-lower reaches of the Han River, which is affected by the Middle Route of the South-to-North Water Diversion Project (SNWDP), the largest inter-basin water transfer project in Asia. We discover that the reversal of the Yangtze River water level could effectively alleviate the occurrence of Han River water blooms. The Yangtze River frequently back flows into the lower reaches of the Han River, with the probability of such events increasing as it nears the confluence (20 km from the Yangtze: 9.5 %, 10 km: 19.0 %, 8 km: 28.6 %). This flow backward carries nutrients that reduce the nitrogen to phosphorus ration (N:P), leading to a shift in the nutrient structure of the Han River. This change is concomitant with a significant decline in algae biomass (Chlorophyll-a = 11.19 µg·L-1 and algae density = 0.41×107 cells·L-1 under natural flow, Chlorophyll-a = 5.19 µg·L-1 and algae density = 0.18×107 cells·L-1 under flow backward), as well as a weakening of the correlation (R) between diatom density and chlorophyll-a concentration, i.e., R = 0.38 (p>0.05) under flow backward conditions versus R = 0.72 (p<0.01) under natural flow conditions. As phosphorus limitation typically suppresses algae growth, the correlation between diatom density and chlorophyll-a concentration can help to reveal the dominance of diatoms, with stronger correlations indicating greater diatom dominance. Consequently, our study provides evidence that the flow backward can alleviate river algal blooms by weakening the growth advantage of diatoms. This study could prove valuable in investigating the eutrophication mechanism within the complex hydrodynamic conditions of rivers. SYNOPSIS: Flow backward caused by the water level difference between the main streams and tributary alleviated the occurrence of river algal blooms in the confluence area.