Synergetic-effect-enhanced electrochemiluminescence of zein-protected Au-Ag bimetallic nanoclusters for CA15-3 detection

In this work, a sandwich-type electrochemiluminescence (ECL) system was constructed for the detection of CA15-3. Gold-silver bimetallic nanoclusters (Au-Ag BNCs) with zein as a protective ligand were synthesized, and the excellent ECL performance of this material was demonstrated for the first time. Zein carrying a variety of groups that ligated with Au-Ag BNCs, forming a protective shell of zein, effectively prevented clusters from aggregating or growing into larger nanoparticles. The synergistic effect of the bimetal promotes the ECL emis-sion, making this nanoscale material an ideal ECL probe. GO-PANI, which effectively promoting the production of sulfate radicals of the co-reactant and significantly increasing the ECL strength, was a good sensing platform for antibody immobilization. Consequently, we constructed an ECL sensor with GO-PANI as the sensing platform and Au-Ag BNCs@zein as the ECL probe, with a detection range of 0.001-100 U mL-1 and a detection limit of 0.0003 U mL-1, provided a strong support for the sensor for future CA15-3 detection applications.