Methods for the purification and detection of single nucleotide KRAS mutations on extrachromosomal circular DNA in human plasma.

Lasse Bøllehuus Hansen, Sandra Fugl Jakobsen,Egija Zole, Julie Boertmann Noer,Li Tai Fang, Sefa Alizadeh,Julia Sidenius Johansen,Marghoob Mohiyuddin,Birgitte Regenberg

Cancer medicine（2023）

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摘要

Taken together our results provide a benchmark for eccDNA purification methods while raising the question of what is required for the optimal fast and sensitive detection of SNP mutations on eccDNA with greater sensitivity than primer-based qPCR detection.

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关键词

circular DNA, eccDNA, KRAS mutations, liquid biopsy, phenol/chloroform DNA extraction, plasmids

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