Creating Optimal Conditions for OPA1 isoforms by Western Blot in Muscle Tissue.

bioRxiv : the preprint server for biology(2023)

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摘要
Samples for western blot analysis are isolated from lysed cells, loaded onto a gel, and ran using optimized conditions to better isolate OPA1 isoforms. Samples are transferred to a membrane to for incubation and protein detection using OPA1 antibodies.
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关键词
opa1 isoforms,skeletal muscle cells,muscle cells,western blot
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