Culturable bacterial endophytes of Aconitum carmichaelii Debx. were diverse in phylogeny, plant growth promotion, and antifungal potential.

Medicinal plants harbor tremendously diverse bacterial endophytes that maintain plant growth and health. In the present study, a total of 124 culturable bacterial endophytes were isolated from healthy Debx. plants. These strains were clustered into 10 genera based on full-length 16S rDNA sequences, among which and were the dominant genera. In addition, may capture 10 potential new bacterial species based on multi-locus sequence analysis of three housekeeping genes (, and ). The majority of these bacterial endophytes exhibited plant growth-promoting ability through diverse actions including the production of either indole acetic acid and siderophore or hydrolytic enzymes (glucanase, cellulose, and protease) and solubilization of phosphate or potassium. A total of 20 strains inhibited hyphal growth of fungal pathogens and on root slices of by the dual-culture method, among which sp. SWUSTb-19 showed the best antagonistic activity. Field experiment confirmed that sp. SWUSTb-19 significantly reduced the occurrence of southern blight and promoted plant biomass compared with non-inoculation treatment. The possible mode of actions for sp. SWUSTb-19 to antagonize against involved the production of glucanase, siderophore, lipopeptides, and antimicrobial volatile compounds. Altogether, this study revealed that harbored diverse plant growth-promoting bacterial endophytes, and sp. SWUSTb-19 could be served as a potential biocontrol agent against southern blight.