Docking guided phase display to develop fusion protein with novel scFv and alkaline phosphatase for one-step ELISA salbutamol detection.

Shuai Hu,Guangbo Yang,Zhou Chen,Qiuye Li, Bin Liu,Ming Liu,Dawei Zhang,Shan Chang,Ren Kong

Frontiers in microbiology（2023）

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摘要

Anti-SAL scFv antibodies with high efficiency were finally identified. In addition, the selected scFv was fused with alkaline phosphatase and expressed in to develop a rapid and low-cost one step ELISA to detect SAL.

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关键词

phage display, docking, salbutamol, scFv, ELISA

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