Glycan cross-feeding drives mutualism betweenFusobacteriumand the vaginal microbiota

SummaryDysbiosis of the vaginal microbiome is associated with vaginal colonization by potential pathogens includingFusobacterium nucleatum, a bacterium linked with intrauterine infection and preterm birth. However, mechanisms by which such pathogens gain a foothold in the dysbiotic vagina remain obscure. Here we demonstrate that sialidase activity, a biochemical marker of vaginal dysbiosis, promotedF. nucleatumforaging on mammalian sialoglycans, an otherwise inaccessible resource. In mice with sialidase-positive vaginal microbiomes, mutantF. nucleatumunable to consume sialic acids displayed impaired colonization. Furthermore, community- and co-culture experiments showed thatF. nucleatumdid not simply take advantage of sialidase-positive bacteria, but also “gave back” to the community, supporting robust outgrowth of sialidase-producers, includingGardnerella vaginalis. These results illustrate that mutualistic relationships between vaginal bacteria support pathogen colonization and reinforce dysbiosis, adding complexity to the simplistic dogma that the mere absence of “healthy” lactobacilli is what creates a permissive environment for pathogens during dysbiosis.