Visualization of basement membranes by a nidogen-based fluorescent reporter in mice

Sugiko Futaki,Ayano Horimoto,Chisei Shimono,Naoko Norioka,Yukimasa Taniguchi,Hitomi Hamaoka,Mari Kaneko,Mayo Shigeta,Takaya Abe,Kiyotoshi Sekiguchi,Yoichi Kondo

Matrix Biology Plus（2023）

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摘要

•Established a mouse model for the basement membrane live imaging.•Recombinant nidogen-1, tagged with a fluorescent protein EGFP or mCherry, labeled the basement membranes both in vitro and in vivo.•The nidogen-based fluorescent probes enable monitoring the dynamics of basement membranes in mouse tissues/organoids.

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关键词

BM,CAG promoter,DAPI,ECM,EGFP,PBS

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