Same-day repeatability and Between-Sequence reproducibility of Mean ADC in PI-RADS lesions

•ADC measurements in prostate lesions show notable variability.•For quantitative ADC assessment, 3D-ROIs are superior to 2D-ROIs.•The cut-off for measurement-based ADC variability is approx. 200 × 10-6 mm2/s (3D-ROIs)•Sequential ADC measurements are feasible by different raters or sequences.•Two DWI sequences demonstrate no significant bias and high comparability.