Relationship between the expression levels of CD4+T cells, IL-6, IL-8 and IL-33 in liver of biliary atresia and postoperative cholangitis, operative age and jaundice clearance

Abstract Objective: To investigate the expression levels of CD4+T cells, IL-6, IL-8,and IL-33 in liver tissue of BA, and the relationship with postoperative cholangitis, operative age and early jaundice clearance.Methods: 45 cases of jaundice treated in hospital from June 2018 to May 2020 were analyzed retrospectively. The expression and distribution of these factors were detected by HE staining and immunohistochemistry, the total bilirubin level and the incidence of cholangitis were recorded, and the relationship between liver inflammation level and the postoperative incidence of cholangitis, age of operation and early jaundice clearance were compared.Results: Immunohistochemistry showed that the expression of CD4+T cells, IL-6, IL-8 and IL-33 in BA group were higher than those in CBD group. ROC curve analysis showed the AUC of CD4+T cells, IL-6 and IL-8 were 0.869, 0.886 and 0.838, respectively. The expression level of CD4+T cells was negatively correlated with the decline rate of TBIL 3 months postoperatively, and the expressions of IL-8 and IL-33 were negatively correlated with the decline rate of TBIL 1 week postoperatively. Conclusion: The high expression of CD4+T cells, IL-6, IL-8 and IL-33 in the BA liver tissue may lead to cholangitis and can be used as a predictor of early jaundice clearance. The degree of liver inflammation infiltration had nothing to do with the age of operation and is not a risk factor for postoperative cholangitis.