A low-cost and robust microscope hardware trigger interface board

In recent years, advancements in microscope design have allowed faster, higher resolution imaging of all types of biological samples. Custom microscope designs have rapidly seen adoption, enabled by a rapid growth in open source software. In this report, we present a custom, open source, hardware design which provides 2 16-bit resolution high-speed analog outputs as well as breakouts for GPIO connections to the inexpensive and well-supported microcontroller (the $4 Raspberry Pi Pico). We provide firmware for interfacing the device with software and demonstrate the device performance when used to interface with a galvanometer. This device provides a working platform for development of custom microscope hardware.