Supplementary Figures S1-S8 from Integrative Genome-Scale Analysis Identifies Epigenetic Mechanisms of Transcriptional Deregulation in Unfavorable Neuroblastomas

Dendrogram and probability values for hierarchical clustering of 105 neuroblastomas based on genome-wide DNA methylation (S1); Kaplan-Meier estimates of overall survival for DNA methylation subgroups (S2); Genes whose hypermethylation and downregulation are associated with neuroblastoma high-risk disease are induced during neuronal differentiation of neuroblastoma cells (S3); Examples for genes whose hypermethylation and downregulation are associated with neuroblastoma high-risk disease and that are marked by both H3K27me3 and DNA methylation at putative regulatory regions in Be(2)-C cells (S4); MYCN deregulation is associated with activation of PRC2 components (S5); Genes whose hypermethylation and downregulation are associated with high-risk disease are H3K27me3-marked in a MYCN-dependent fashion (S6); Example for genes whose hypermethylation and downregulation are associated with neuroblastoma high-risk disease that significantly lost H3K27me3 and DNA methylation at putative regulatory regions upon treatment with DAC and EPZ-6438 in Be(2)-C cells (S7); Genes whose hypermethylation and downregulation are associated with high-risk disease are preferentially induced upon DAC/TSA treatment (S8).