Supplementary Material from C-RAF Mutations Confer Resistance to RAF Inhibitors

Supplementary Material - PDF file 1876K, Supplementary Figure S1. Characterization of mutants obtained from B-RAFV600E random mutagenesis screens Supplementary Figure S2. Biochemical characterization of C-RAF resistance alleles Supplementary Figure S3. Effects of MEK inhibition on C-RAF resistance mutants Supplementary Figure S4. 293/T cells coexpressing His/V5 tagged C-RAF resistance mutants with Flag tagged WT-C-RAF were immunoprecipitated after 48 h with His and levels of Flag tagged C-RAF interaction was assessed by immunoblotting. Input lysate represents Flag-C-RAF, V5-C-RAF, C-RAF, p-MEK, p-ERK and total ERK. Supplementary Figure S5. A model of the putative effects of pharmacological RAF inhibitors on C-RAF resistance alleles. Supplementary Figure S6. Integrated Genomics Viewer (IGV) image of a RAF1 mutation present in a primary melanoma tumor