Identification of U p 47 in three thermophilic archaea, one mesophilic archaeon, and one hyperthermophilic bacterium.

Analysis of the profile of the tRNA modifications in several allowed us to observe a novel modified uridine in the V-loop of several tRNAs from two species: and Recently, Ohira and colleagues characterized 2'-phosphouridine (U) at position 47 in tRNAs of thermophilic , as well as in several other archaea and thermophilic bacteria. From the presence of the gene corresponding to the RNA kinase responsible for U47 formation, they also concluded that U47 should be present in tRNAs of other thermophilic Reanalysis of our earlier data confirms that the unidentified residue in tRNAs of both and is indeed 2'-phosphouridine followed by mC48. Moreover, we find this modification in several tRNAs of other and of the hyperthermophilic bacterium .