Epithelial-mesenchymal transition as a potential route for DAPT resistance in breast cancer cells

Notch is a conserved pathway involved in cell-fate determination and homeostasis. Its dysregulation plays a role in poor prognosis and drug resistance in breast cancer. Targeting Notch signaling via inhibition of the gamma-secretase complex is in the spotlight of modern cancer treatments. Gamma-secretase inhibitors (GSI) have shown successful clinical activity in treating cancers, yet the possible resistance mechanism remains unstudied. Modeling the resistance and understanding culprit molecular mechanisms can improve GSI therapies. Accordingly, the aim of this study is to generate and analyze GSI-resistant breast cancer cells.