Figure S1, Figure S2, Figure S3, Figure S4, Figure S5 from Identification of New Tumor Suppressor Genes in Triple-Negative Breast Cancer

Figure S1. Expression of ZNF326 a in human breast cancer tissues. The images show a representative scoring intensities following immunostaining with anti-ZNF326 antibody (Range of low, moderate and high). Figure S2. RT-PCR analysis of the candidate cancer genes downregulated by shRNA-pools in the (A) HCC70 and (B) MDA-MB-231 breast cancer cell lines. Figure S3. Stable ZNF326 knockdown in (A) HCC70, (B) HCC1569 and (C) MDA-MB-231 TNBC cell lines using two independent shRNAs was confirmed by qRT-PCR. (D) Ectopic expression of ZNF326 in MDA-MB-231 cells transduced with lentivirus particles expressing ZNF326 cDNA and empty vector (control) respectively. ZNF326 mRNA levels were quantified by qRT-PCR. Figure S4. Western blot analysis of mammosphere cell lysates of HCC70 (A) and MDA-MB-231 (B) with stable shRNA (left panel) and ectopic overexpressing ZNF326 (right panel). Figure S5. Gene expression-based outcome for breast cancer online (GOBO) was used to obtain the expression analysis of (A) DACH1 and (B) GATA3 in different breast cancer subtypes.