Synthesis and biological evaluation of benzoxepinoindol-1-one analogs as Brd4 bromodomain inhibitors

A novel series of benzo[6,7]oxepino[4,3,2-cd]indol-1(2H)-one derivatives were synthesized via a one-pot aldol condensation and SNAr reaction by coupling indolin-2-ones with 2-fluorobenzaldehydes. In addition, molecular docking studies of the designed compound 2 revealed strong hydrogen bonds in the hot binding pocket of Brd4. All compounds were evaluated for their enzymatic activity in Brd4 bromodomain inhibition.