CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

Rubina Tuladhar,Yunku Yeu,John Tyler Piazza,Zhen Tan,Jean Rene Clemenceau,Xiaofeng Wu,Quinn Barrett,Jeremiah Herbert,David H. Mathews,James Kim,Tae Hyun Hwang,Lawrence Lum

Nature Communications（2019）

引用 108|浏览1

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摘要

CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.

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