Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains

Dongjin R Lee,Yajun Zhang,Christopher T Rhodes,Timothy J Petros

STAR Protocols（2023）

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摘要

Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modified for any brain region and/or neural cell type.

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关键词

Single Cell,Flow Cytometry/Mass Cytometry,Developmental biology,Sequencing,RNAseq,Neuroscience,Cell Differentiation

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