Evaluation of Vibration-Induced flow patterns for reproducible cell spheroid production

In recent years, cell spheroids have attracted a great deal of attention for their applications in tissue engineering, such as drug efficacy evaluation and organ and blood vessel regeneration. Previously, we proposed a method for generating cell spheroids using vibration-induced flow. However, repeatability of generating cell spheroids was low. Thus, in this paper, we discuss the suitable design of flow pattern induced around micropillar to improve the repeatability of cell spheroids fabrication. By comparing the results of cell aggregation and the flow patterns, we confirmed the swirly flow patterns related to the formation of cell spheroids with high repeatability.