2D Co metal-organic framework nanosheet as an oxidase-like nanozyme for sensitive biomolecule monitoring

Nanozyme-based biomolecules sensitive and quantitative detection is an attractive strategy due to their high chemical, thermal stability and reactive activity. Here, we have synthesized a significant number of two-dimensional (2D) cobalt-metal-organic framework (Co-MOF) nanosheets with oxidase (OXD)-like activity using a facile solvothermal method in one pot for biomolecule monitoring. The synthesized Co-MOF nanosheets exhibit strong stability, higher specific surface area and more active sites due to their MOF structure. Such Co-MOF nanosheets with excellent OXD-like activity show adequate analytical performance in the quantitative determination of dopamine (DA) and glutathione (GSH) with a wider dynamic sensing range and lower detection limits (DA and GSH: 0.24 and 0.067 μmol·L −1 , 3 σ /slope, where σ is standard deviation of the blank). This work extends the application of 2D-MOF structures in bioassays and brings new insights into the application of OXD-like nanozymes in bioassays. Graphical abstract