Enhancing Two-Photon Images for Anatomical Visualisation using Super-Resolution

Two-photon Laser Scanning Microscopy (2P-LSM) is a technique used to image the living tissue with relatively high spatio-temporal resolution. However, the time-series images are often corrupted with Poisson-Gaussian noise and deteriorated with motion artifacts. This paper deals with the problem of enhancing 2P-LSM images to reconstruct high quality and high spatial-resolution outputs using the observed time-series stack of low-resolution images. The proposed technique consists of several components including noise filtering, image registration, cell detection and focus measurement, and clustering for a joint denoising and super-resolution. Extensive experiments demonstrate that the proposed method results in gratifying output images containing apparent and clear cell forms at different focus levels.