Sensitive and rapid RT-RPA-Cas12a-mediated detection method capable of human rhinovirus A and/or C species by targeting VP4

Weidong Qian,Xuefei Wang,Jie Huang, Jian Liu,Si Chen,Ting Wang,Dandan Zhang,Yongdong Li

Virus Research（2023）

引用 1|浏览8

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摘要

•The fluorescence or LFS assay based on RT-RPA-Cas12a is a point-of-care diagnosis method.•The fluorescence or LFS assay based on RT-RPA-Cas12a detection can be completed within 50 min with high specificity.•The RT-RPA-Cas12a mediated fluorescence or LFS assay demonstrates high sensitivity (0.1 or 0.5 copy per microliter).

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关键词

Human rhinovirus,Reverse-transcription recombinase polymerase amplification,CRISPR-Cas12a,Detection,Rhinovirus species A or C

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