Local methods to improve cryo-electron microcopy maps

Satinder Kaur,J. Gomez-Blanco,Ahmad Abdelzaher Zaki Khalifa,Swathi Adinarayanan,Rubén J. Sánchez-García,Daniel Wrapp,Jason S. McLellan,Khanh Huy Bui,Javier Vargas

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)（2021）

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摘要

Cryo-electron microscopy (cryo-EM) has become a powerful technique for protein structure determination. However, there are still important issues to be addressed, such as, robust sharpening or local B-factors estimations. To address these issues, we present here new local methods to improve the analysis and interpretability of cryo-EM maps.

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