Crossing Plasmopara viticola strains in controlled conditions to uncover the genomic bases of downy mildew resistance breakdown in grapevine

The biotrophic oomycete Plasmopara viticola is the causal agent of downy mildew, one of the major grapevine diseases. We report here a successful strategy to cross compatible strains of this pathogen and obtain a large viable progeny. We used this method to study the offspring between two P. viticola strains able to overcome two major grapevine resistance factors ( Rpv3 , Rpv10). Thanks to the genomic resources now available, we will genotype this progeny to build an unprecedented linkage map and uncover the genomic bases of grapevine resistance breakdown displayed by P. viticola virulent strains.