Bioorthogonal site-selective conjugation of fluorescent dyes to antibodies: method and potential applications

Philipp Grossenbacher,Maria C. Essers, Joël Moser,Simon A. Singer,Stephanie Häusler,Bruno Stieger,Jean-Sébastien Rougier,Martin Lochner

RSC Advances（2022）

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摘要

Several synthetic fluorescent dibromopyridazinediones were conjugated site-selectively to IgG1 antibodies to generate imaging tools for western blot and immunofluorescence applications.

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关键词

fluorescent dyes,antibodies,conjugation,site-selective

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