Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications

llustration of a novel drug-loaded and surface functionalized protein/nisin shelled nanoemulsion (NSNE). (A) doxorubicin loaded into the oily core of the nanovesicles (B) Bifunctional amine-reactive and SPAAC reactive linker coupling to the lysine residue on the Nisin shell (C) Site-specific Sortase A mediated Azide Bioconjugation (SMAB) to a targeting antibody using a G-PEG-N3 linker (D) Biorthogonal click reaction (SPAAC) for particle assembly.