Abstract B009: Establishing new cell lines from undifferentiated pleomorphic sarcoma for sarcoma research

Abstract Undifferentiated pleomorphic sarcoma (UPS), also known as malignant fibrous histiocytoma (MFH), is a high-grade soft-tissue sarcoma (STS). Despite of importance, there are very few models for study. Here, we have established and characterized UPS-derived cell lines. Cells were obtained UPS tissues by mincing followed by extracting or dissociating using enzymes and cultured by regular culture environment. Cells were maintained and immortal for months without artificial treatment and tumorigenic in vivo study. The tissues from in vivo study and tissues from patients were compared if it is representative for UPS by immunohistochemistry. Transcriptome from tissues and cell lines were compared. Several genes were identified as specific in tumor tissues and cell lines. Fusion genes were also identified. This study showed that new UPS cell lines might be a good resource for UPS study to get new insights. Citation Format: Hye Jin You, Eun-Young Lee, Abu Rayhan, Hyun Guy Kang, June Hyuk Kim, Jongwoong Park. Establishing new cell lines from undifferentiated pleomorphic sarcoma for sarcoma research [abstract]. In: Proceedings of the AACR Special Conference: Sarcomas; 2022 May 9-12; Montreal, QC, Canada. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(18_Suppl):Abstract nr B009.