Application of subject-specific helmets for the study of human visuomotor behavior using transcranial focused ultrasound: a pilot study

•We proposed the framework of transcranial focused ultrasound (tFUS) stimulation integrating our two previously proposed methods for tFUS transducer configuration optimization and a subject-specific 3D-printed helmet.•To validate this framework, tFUS stimulations were applied to the left dorsolateral prefrontal cortex (DLPFC) while human participants were performing anti-saccade tasks.•The result indicates that the cortical stimulation using our tFUS setup effectively reduces the error rates of anti-saccade tasks, whereas no significant effect was observed on their latencies.•We demonstrated that our tFUS framework can be effectively used in human behavioral study, and our tFUS stimulation targeted to the DLPFC could generate a neuromodulatory effect on AS behavior.