A tripartite-enzyme via curcumin regarded as zymoexciter towards highly efficient relieving reperfusion injury

For alleviating ischemic reperfusion injury, reasonable design mimic multi-functional nanozyme can simulta-neously capture ROS and inhibit inflammatory factors, which plays an important role. However, the low effi-ciency of natural enzyme and their ability to capture only a single species limit their effectiveness. Therefore, additional materials to capture other ROS are highly needed. These motivates us to construct a nanozyme via hybridize/coupled curcumin (CUR) with ZnCe Layered Rare-earth Hydroxide (ZnCe-LRH) to achieve highly efficient ROS scavenging efficiency and the multiple ROS capture (denoted as CUR/ZnCe-LRH). Ce element was highly dispersed on the LRH layer, and CUR further coordinated with Zn on the layer to form a stable nanozyme structure. This mimic tripartite nanozyme can not only simulate superoxide dismutase (SOD) and catalase (CAT) activity to capture center dot & nbsp;O-2(-)& nbsp;and H2O2, but also arrest center dot OH within a short period of time, which can be regarded as the tripartite nanozyme. Density functional theory (DFT) calculations can further confirm the high efficient ROS scavenging mechanism by the CUR/ZnCe-LRH systems. In vivo results can further probe that this CUR/ZnCe-LRH can also suppress inflammation-and immune response-induced injury, thus achieving good prevention and treatment in neuroprotective therapy. The infarct area can reduce by 78% after the reperfusion therapy and the neurological deficit score can reduce from 3.50 to 0.67. This strategy provided a novel multifunctional enzyme mimetic for ischemia-reperfusion therapy and clarifies the application mechanism of neuroprotection against ischemia-reperfusion injury.