Separation of unsaturated C18 fatty acids using perfluorinated-micellar electrokinetic chromatography: II) role of nano-structuration

•Free fatty acids are separated by capillary electrophoresis using fluorosurfactants.•Scattering experiments evidence aggregation from small micelles to large structures.•Micelle structuration in injection buffer and background electrolytes is affected by fatty acids.•Selective interactions between fatty acids and fluorosurfactant allow CE separation.