Temperature sensitivity of soil enzyme kinetics under N and P fertilization in an alpine grassland, China.

Soil nutrient cycling can be best studied by supplementing the soil with N and P fertilizers. Soil enzyme kinetic parameters (Vmax and Km) can be used to reflect the maximum reaction rates and affinities of soil enzymes. However, how N and P fertilizers affect the temperature sensitivity of soil enzyme kinetics is poorly understood. Therefore, our study investigated the response of soil enzyme kinetic temperature sensitivity relevant to C, N, and P cycles based on a 9-year fertilization (N and P) experiment performed in an alpine grassland on the Qinghai-Tibetan Plateau in China. Our results showed the following: N and P addition positively affected the Km of β-glucosidase (BG); P and NP interaction significantly increased the Km of phosphatase (AP), indicating that N and P addition significantly negatively affected the substrate affinity of soil enzymes. The temperature sensitivity of Michaelis-Menten kinetics was different for different enzymes. N and P fertilization decreased the temperature sensitivity of Km of BG but increased the temperature sensitivity of the Km of N-acetyl-glucosaminidase (NAG) and AP. In our study, P and NP fertilization increased the temperature sensitivity and activation energy of the Vmax of BG, indicating that P elements promoted the secretion of more extracellular enzymes by soil microbes to cope with temperature changes. The enzymes involved in the soil N and P cycle responded to the exogenous N and P through increases and decreases in the temperature sensitivity of the Km and Vmax, respectively. This study is crucial for investigating the impact of nutrient input on soil ecosystem functions under future climate warming conditions.