Brevibacillus dissolubilis sp. nov., Isolated from Fresh Water

A Gram-positive-staining, strictly aerobic, motile, ellipsoidal endospore-forming bacterial strain, designated CHY01 T , was isolated from the Chishui river in a section of Maotai Town, Guizhou Province, Southwest China. Strain CHY01 T was found to grow optimally at pH 8.0 and 28 °C. The 16S rRNA gene sequence analysis indicated that strain CHY01 T belonged to the genus Brevibacillus and clustered with the type strain of Brevibacillus panacihumi , with which it exhibited 16S rRNA gene sequence similarity values of 97.8%. The predominant respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were C 14:0 , iso-C 15:0 , anteiso-C 15:0 , C 16:0 , C 15:1 iso-H and/or C 13:0 3-OH, and C 16:1 ω 7 c and/or C 16:1 ω 6 c . Genome sequencing revealed a genome size of 6.1 Mbp and a G + C content of 50.6%. The results of physiological and biochemical tests allowed strain CHY01 T to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Pairwise determined whole-genome average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values suggested that strain CHY01 T represents a new species, for which we propose the name Brevibacillus dissolubilis sp. nov. with the type strain CHY01 T (= CGMCC 1.15916 T = KCTC 33863 T ).