Association of Bridging Integrator 1 Gene Polymorphism with Susceptibility to Alzheimer’s Disease

Background: Alzheimer's disease (AD) is the most common type of dementia. The role of genetic factors in the development of AD has not been demonstrated yet. Objectives In this study, our aim was to investigate the association between one of the BIN1 gene’s single-nucleotide polymorphism (SNP( (rs744373) and late-onset Alzheimer's disease (LOAD) in an Iranian population in the Guilan province. Materials & Methods: In this case-control study, 110 patients with LOAD, and 110 unrelated healthy controls were recruited. Polymerase chain reaction-restriction length polymorphism (PCR-RFLP) was performed for genotyping BIN1 gene’s SNP (rs744373). Electrophoresis was there after carried out using agarose gel and DNA safe stain and the gels were visualized under an ultraviolet (UV) trans-illuminator. The allelic and genotypic frequencies were determined. Results: The frequency of allele T (Wild-type allele) in the control and the LOAD groups was 70.9% (n = 159) and 58.6% (n = 129), respectively (p = 0.007). The frequency of allele C in the LOAD group (41.4%) (n = 91) was significantly higher than that of the control group (29.1%) (n=64) (p=0.007). Frequency of homozygous genotype (CC) of BIN was significantly higher in the LOAD group than in the control group (p = 0.043). Conclusion: rs744373 SNP of BIN1 gene is significantly associated with the risk of developing AD in the studied population.