Chiral Ammonium Salt Ion Pairing Organocatalysis

I will introduce our recent additions to advance the stable isotope (SI) labelling procedures for nucleic acids and how we use these tailor-made SI labelled RNAs and DNAs in NMR spectroscopic applications. As a recent example the F-C-aromatic labelling of RNA nucleobases to unlock the TROSY effect in RNA will be presented. Further, synthetic routes will be presented, which give access to chemical building blocks of naturally occurring RNA modifiers, such as, 6-methyl adenosine (mA). These modified building blocks were further labelled with nuclear magnetic resonance (NMR) active C or N stable isotopes. Thereby, detailed NMR investigations on the effects of the modifiers on structural and dynamic features of RNA were possible. In detail, the effect of the mA modification on the annealing properties of RNA will be presented. Text