Comparison of two techniques used for the evaluation of point-of-care methodology for testing hemoglobin in blood donors

Background and Objectives: Precise measurement of Haemoglobin(Hb) is necessary before the blood donation procedure for donor's protection against anemia and to ensure blood elements' quality to ensure the better effect of these blood constituents on the clinical outcomes of recipients. This is a comparative analysis of DiaSpect Tm and AccuSure hemoglobin (Hb) for point-of-care technique (POCT) for testing of Hb and to evaluate their effectivness by using venous blood as sample and Sysmex XP100 cell counter as the reference method. Methods: A total of 544 samples of blood donors between the age group 18 and 65 years were taken for this study. Measurement of Hb was done with the use of three different Hb analyzers – DiaSpect Tm, AccuSure Hb (Model HB101), and Sysmex XP100 Cell counter by using venous blood samples. P < 0.001 was taken statistically significant. Results: AccuSure Hb was found to have lower sensitivity in Hb estimation in the donor's blood compared to that of the DiaSpect Tm analyzer. Among various available techniques, there is a requirement to find a more appropriate technique for the estimation of Hb in the donor's blood. Conclusion: We found that DiaSpect Tm has greater sensitivity in comparison to AccuSure Hb. Furthermore, specificity, positive predictive value, negative predictive value, and accuracy of DiaSpect Tm were greater in comparison to those of AccuSure Hb.