Sutcliffiella deserti sp. nov., isolated from desert soil

A Gram-stain-positive, facultative anaerobic, motile and rod-shaped bacterial strain. DG-18(T), was isolated from desert soil sampled at the Kubuqi Desert in Inner Mongolia, China. Strain DG-18(T) grew at 4-40 degrees C (optimum. 25-30 degrees C), at pH 8.0-10.0 (optimum, pH 9.0) and with 0-8.0% (w/v) NaCl (optimum 2.0%). 16S rRNA gene sequence analysis placed strain DG-18(T) within the genus Sutcliffiella of the family Bacillaceae with Sutcliffiella halmapala DSM 8723(T) (98.2%), Sutcliffiella zhanjiangensis JSM 099021(T) (97.6%), Sutcliffiella horikoshii DSM 8719(T) (97.4%), Sutcliffiella catenulata 18C(T) (96.6%) and Sutcliffiella cohnii NBRC 15565(T) (96.5%) as its closest relatives. The major respiratory quinone of strain DG-18(T) was MK-7 and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Its major fatty acids were iso-C(15)(:0)( )and iso-C-17:1 omega 10c. The genomic DNA G+C content of strain DG-18(T) was 38.7mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain OG-18(T) and that of S. halmapala DSM 8723(T) was 76.7%. The Genome-to-Genome Distance Calculator showed that the DNA-DNA hybridization value for strain DG-18(T) and S. halmapala DSM 8723(T) was 21.8%. Based on the polyphasic taxonomic data, strain DG-18(T) represents a novel species of the genus Sutcliffiella, for which the name Sutcliffiella deserti sp. nov. is proposed. The type strain is DG-18(T )(=GDMCC 1.17773(T)=KCTC 431701(T)).