Nanoengineered Light-Harvested Proteins for Optogenetics and Photopharmacology
semanticscholar(2019)
摘要
Chemical modification with nanometer
precision can be used to probe and to improve the function of complex molecular
entities, from organic materials to proteins and their assemblies. Using the
pigment arrangement in photosynthetic light-harvesting as inspiration, we show
that molecular photosensitizers can be located at well-defined distances from
photoisomerizable units in proteins in order to enhance and spectrally shift their
photoresponses. The approach is demonstrated in Channelrhodopsin-2 (ChR2) and
in the light-gated ionotropic glutamate receptor (LiGluR), two archetypical
actuators in optogenetics and photopharmacology that have been used both for
fundamental and therapeutic purposes. These proof-of-concept experiments together
with theoretical simulations predict that the photosensitivity can be increased
several orders of magnitude using these means, thus providing a unique
methodology to boost the performance of current optogenetic and
photopharmacological toolboxes.
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