Multiplex SARS-CoV-2 RT-qPCR protocol v1

Peter H L Krijger,Tim A Hoek,Sanne Boersma,Lieke I P M Donders, Maaike M C Broeders,Mark Pieterse, Pim W Toonen,Ive Logister,Bram M P Verhagen,Marjon J A M Verstegen,Thomas W van Ravesteyn,Rene J T M Roymans,Francesca Mattiroli,Jo Vandesompele,Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker,Fred J Dom, Rob Ruijtenbeek, Lieven B J van der Velden,Jeroen H B van de Bovenkamp, Martijn Bosch,Wouter de Laat,Marvin E Tanenbaum

semanticscholar（2021）

引用 0|浏览3

暂无评分

摘要

An in-house multiplex RT-qPCR , targeting SARS-CoV-2and PDV as internal control [1][2], developed on QuantStudio 7 Pro Real-Time PCR Systems using Life Technologies Taqman FastVirus 1-step mastermix with E-gene primers and probe as described by Corman et al. and N1 primers and probes as described by the CDC[3, 4]. 1.Clancy, A. eta al., The development of a qualitative real-time RT-PCR assay for the detection of hepatitis C virus European Journal Microbial Infectious Diseases, 2008. 276(12): p.1177. 2.Wolters, F., et al., Multi-center evaluation of cepheid xpert® xpress SARS-CoV-2 point-of-care test during the SARS-CoV-2 pandemic. Journal of Clinical Virology, 2020. 128: p. 104426 3.Corman, V.M., et al., Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill, 2020. 25(3). 4.Lu, X., et al., US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2.Emerging Infectious Disease journal, 2020. 26(8): p. 1654.

查看译文

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要