Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis)

Ruiyuan Liu,Xicun Dong,Miaomiao Zhang,Ying Qu,Wenjie Jin,Xin Li,Libin Zhou,Lixia Yu,Wenjian Li

Scientia Horticulturae（2021）

引用 7|浏览16

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摘要

•Altered levels of chlorophyll and anthocyanin contents, and abnormal ultrastructural features of chloroplasts were detected in the thermo-sensitive leaf-color mutant of green wandering Jew.•RNA-Seq analysis identified 42 candidate genes involved in photosynthesis, 14 candidate genes for chlorophyll biosynthesis, and 13 candidate genes for flavonoid and anthocyanin biosynthetic pathways.•Chlorophyll fluorescence signals, and HPLC confirmed the gene expression levels obtained by RNA-Seq.

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关键词

Green wandering Jew,Leaf-color mutant,Temperature,Transcriptome,Flavonoids,Photosynthesis,Abbreviations: ANR, anthocyanidin reductase,ANS, anthocyanin synthase,Ant, anthocyanin,AP2/ERF, APETALA2/ethylene-responsive factor,atp, ATP synthase,bHLH, basic helix-loop-helix,Chl, chlorophyll,ChlH, magnesium chelatase H subunit,ChlM, Mg protoporphyrin methyltransferase,CHI, chalcone flavanone isomerase,CHS, chalcone synthase,DEGs, differentially expressed genes,DFR, dihydroflavonol 4-reductase,FDR, False Discovery Rate,FLS, flavonol synthase,FPKM, fragments Per Kilobase of exon model per Million mapped fragments,F3’H, flavanone 3’-hydroxylase,F3’5’H, flavanone 3’5’-hydroxylase,HemA, glutamyl-tRNA reductase,HemB, porphobilinogen synthase,HemC, hydroxymethylbilane synthase,HPLC, high-performance liquid chromatography,LAR, leucoanthocyanidin reductase,KEGG, the Kyoto Encyclopedia of Genes and Genomes,LET, linear energy transfer,Lhc, light-harvesting chlorophyll complex,mt, thermo-sensitive leaf-color mutant,MYB, v-myb avian myeloblastosis viral oncogene homolog,NGS, next-generation sequencing,Pet, photosynthetic electron transport,Por, protochlorophyllide oxidoreductase,PS Ⅰ, photosystem Ⅰ,PS Ⅱ, photosystem Ⅱ,qRT-PCR, quantitative reverse transcription polymerase chain reaction,RNA-seq, RNA sequencing,RPKM, reads per kb per million reads,TEM, transmission electron microscopy,TFs, transcription factors,UFGT, UDP-glucose: flavonoid 3 - O - glucosyltransferse,WRKY, WRKY transcription factor,WT, wild type

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