The Influence Of Autoblood Treated With An Improved Preservation Solution On The Wound Healing Of Diabetes Mellitus Mouse Models And The Functional Mechanism Of The Hif-1 Alpha Signaling Pathway In Wound Healing

Objective: This paper aims to analyze the promotion effect of autoblood treated with an improved preservation solution (IPS) on the wound healing (WH) of diabetes mellitus (DM) mouse models. Methods: 60 healthy male SD mice were selected as research materials and divided into a standard group (SG), which included 20 mice, an improved group (IC), which included 20 mice, and a blood preparation group (BPG), which included 20 mice, after building the DM mouse models so as to compare the WH in the three groups. The SG was transfused with autoblood treated with a standard preservation solution (SPS), the IG was transfused with autoblood treated with IPS, and the BPG was respectively kept in vitro of the IPS and SPS for one week. Results: (1) The wound healing rate (WHR) of the IG was higher than it was in the SG at 1 d, 4 d, 7 d, and 14 d after the autologous blood transfusion (ABT) (P<0.05). (2) Using real-time fluorescent quantitative PCR (RT-qPCR), we found that the mRNA expression levels of HIF-1 alpha, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and Hsp90 in the IG were higher than they were in the SG (P<0.05). (3) Using immumohistochemical staining (IHC staining), we found that the positive expression rates (PER) of HIF-1 alpha, VEGF, and EGF in the IG were higher than they were in the SG (P<0.05). (4) After silencing the HIF-1 alpha in the fibroblasts using siRNA, the measurement results based on the Western blot method showed that the protein expression levels of HIF-1 alpha, VEGF, and MMP-2 in the IG were higher than they were in the SG (P<0.05). (5) According to the CCK8 quantification, the fibroblast viability (FV) of the IG was higher than it was in the SG at 24 h, 48 h, and 72 h after the ABT (P<0.05). (6) According to the quantification using flow cytometry (FCM), the cell quantity (CQ) of the IG was much higher than it was in the SG in the G0/G1 stages and much lower than it was in the SG in the G2/M stage (P<0.05), and there was no significant difference in the CQ between the IG and the SG in the S stage (P>0.05). Conclusion: Autoblood treated with IPS accelerated the WH of the DM mouse models, and the activated HIF-1 alpha signaling pathway regulated the FV to enhance the cell viability, strengthen cell proliferation and migration, and thus accelerate the WH.