The In Vitro And In Vivo Effects Of Cisplatin On Growth Of Oral Cancer Cells Regulated By Mir-633 And Mir-210

The objective of this study was to explore the effects of cisplatin on growth of human oral cancer cells, and the differential expression of two micro RNAs (miRs), miR-633 and miR-210 in vitro and in vivo. The growth-inhibiting effects of cisplatin on the human oral cancer cell line SCC25 were assessed by MTT assay, while cell apoptosis was examined using DAPI staining. In addition, the effects of cisplatin on the growth of oral cancer in a murine model were tested and the expressions of miR-633 and miR-210 were quantified using real-time PCR. We observed that, cisplatin significantly decreased the growth of human oral cancer cells in vitro (P < 0.05 vs. control cells) at both 24 and 48 hours of incubation, with effects being more pronounced at the latter time point. Furthermore, Microscopic studies demonstrated apoptotic signs in cispatin-treated cells with a clear dose-response pattern. In the murine tumor model, tumor volumes were smaller in cisplatin-treated animals, tumor weight were reduced in cisplatin-treated animals. Subsequently, the miR-633 was down-regulated, while miR-210 was up-regulated by cisplatin. Thus, cisplatin significantly inhibits proliferation of oral cancer cells, and the effects are associated with down-regulation of miR-633 expression and up-regulation of miR-210 expression.