Aurantio-obtusin induces hepatotoxicity through activation of NLRP3 inflammasome signaling

•AO delays yolk sac absorption, and increases liver area and inflammation in larval zebrafish.•AO is quickly absorbed into the blood and rapidly metabolized in mice.•AO induces hepatotoxicity in a gender-dependent manner in mice.•AO induces liver dysfunction, hepatocyte necrosis with inflammation in female mice.•AO activates NLRP3 inflammasome signaling via increasing KCNN4 and ROS and inhibiting NF-κB.