Elevated Fibronectin Levels In Profibrotic Cd14(+) Monocytes And Cd14(+) Macrophages In Systemic Sclerosis

Background: Systemic sclerosis (SSc) is an autoimmune disease characterized by overproduction of extracellular matrix (ECM) and multiorgan fibrosis. Animal studies pointed to bone marrow-derived cells as a potential source of pathological ECM-producing cells in immunofibrotic disorders. So far, involvement of monocytes and macrophages in the fibrogenesis of SSc remains poorly understood.Methods and Results: Immunohistochemistry analysis showed accumulation of CD14(+) monocytes in the collagen-rich areas, as well as increased amount of alpha smooth muscle actin (alpha SMA)-positive fibroblasts, CD68(+) and mannose-R+ macrophages in the heart and lungs of SSc patients. The full genome transcriptomics analyses of CD14(+) blood monocytes revealed dysregulation in cytoskeleton rearrangement, ECM remodeling, including elevated FN1 (gene encoding fibronectin) expression and TGF-beta signalling pathway in SSc patients. In addition, single cell RNA sequencing analysis of tissue-resident CD14(+) pulmonary macrophages demonstrated activated profibrotic signature with the elevated FN1 expression in SSc patients with interstitial lung disease. Peripheral blood CD14(+) monocytes obtained from either healthy subjects or SSc patients exposed to profibrotic treatment with profibrotic cytokines TGF-beta, IL-4, IL-10, and IL-13 increased production of type I collagen, fibronectin, and alpha SMA. In addition, CD14(+) monocytes co-cultured with dermal fibroblasts obtained from SSc patients or healthy individuals acquired a spindle shape and further enhanced production of profibrotic markers. Pharmacological blockade of the TGF-beta signalling pathway with SD208 (TGF-beta receptor type I inhibitor), SIS3 (Smad3 inhibitor) or (5Z)-7-oxozeaenol (TGF-beta-activated kinase 1 inhibitor) ameliorated fibronectin levels and type I collagen secretion.Conclusions: Our findings identified activated profibrotic signature with elevated production of profibrotic fibronectin in CD14(+) monocytes and CD14(+) pulmonary macrophages in SSc and highlighted the capability of CD14(+) monocytes to acquire a profibrotic phenotype. Taking together, tissue-infiltrating CD14(+) monocytes/macrophages can be considered as ECM producers in SSc pathogenesis.