Triplex Direct Quantitative Polymerase Chain Reaction For The Identification Of Streptococcus Pneumoniae Serotypes

Mahamoudou Ouattara,Mamadou Tamboura,Dinanibè Kambiré,Kim Anh Lê,Thanh Van Phan,Srinivasan Velusamy,Hien Anh Nguyen,Dai Vo Thi Trang,Fernanda C Lessa, Makiko Iijima,Dac Trung Nguyen,Stephanie B Schwartz,Lesley McGee,Rasmata Ouédraogo Traoré,Bernard Beall

JOURNAL OF INFECTIOUS DISEASES（2021）

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摘要

The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.

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关键词

Streptococcus pneumoniae, direct real-time PCR, pneumococcal serotype

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