Streptomyces luteolifulvus sp. nov. , a novel actinomycete isolated from soil in Nanjing, China

During the investigation of exploring potential sources of novel species and natural bioactives, a novel actinomycete, designated strain HIT-DPA4 T , was isolated from a soil sample, which was collected from Nanjing, Jiangsu Province, PR China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and the result of phylogenetic analysis, strain HIT-DPA4 T was most closely related to Streptomyces cyaneus CGMCC 4.1671 T , and shared the highest sequence similarity of 98.76%. In addition, the cell walls of the species HIT-DPA4 T contained LL-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were identified as glucose and ribose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and phosphatidylmonomethylethanolamine. MK-9(H 6 ) and MK-9(H 4 ) were predominant menaquinones; and C 16:0 , anteiso-C 15:0 and C 15:0 as major cellular fatty acids of the organism HIT-DPA4 T . Gene Ontology database analysis and antiSMASH server predicted results displayed that strain HIT-DPA4 T was a promising classification units, which has various types of functions and contains multiple biosynthetic gene clusters with the similarity more than 80%. Multilocus sequence analysis (MLSA) of five housekeeping genes ( atp D, gyr B, rec A, rpo B and trp B) illustrated that Streptomyces luteolifulvus formed a separate branch in the genus Streptomyces . However, a combination of low level of DNA-DNA relatedness and physiological properties indicated that strain HIT-DPA4 T can be distinguished from its phylogenetically related species Streptomyces cyaneus CGMCC 4.1671 T . Moreover, gene synteny research could be further differed organism HIT-DPA4 T from similarity species. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces , for which the name Streptomyces luteolifulvus sp. nov. is proposed. The type strain is HIT-DPA4 T (= CGMCC 4.7558 T = TISTR 2751 T ).