Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis.
STAR protocols(2021)
摘要
High cell viability and recovered cell concentration are typical quality control requirements for single-cell processing and quality data. This protocol describes procedures for sampling, live-cell biobanking, preprocessing for single-cell RNA sequencing, and analysis of fine-needle aspiration (FNA) samples of the skin. The minimally invasive nature of FNA collection is more accepted by patients and allows for frequent longitudinal sampling, resulting in high-quality single-cell sequencing data that capture cellular heterogeneity in clinical samples.
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关键词
Bioinformatics,Cell isolation,Clinical Protocol,RNAseq,Single Cell
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