Novel Rolling Circle Amplification Biosensors For Food-Borne Microorganism Detection

Rolling circle amplification (RCA) technology provides a better alternative for the highly efficient, visual, and rapid on-site detection and poisoning mechanism screening for food-borne microorganisms. The review focuses on the innovative analytical strategies of six primary kinds of microbial targets, including one nucleic acid target and five non-nucleic acid targets (pathogens, proteins, lipopolysaccharides, mycotoxins, and bacterial toxins), covering four recognition elements of antibody, aptamer(Apt), probe and primer, and introduces the latest analysis strategies. These innovative sensing strategies are sum-marized from five perspectives for the first time, including the combination of RCA technology and immune reactions, functional nucleic acids (FNAs), nanomaterials, multiple amplification technologies, and portable devices, fully demonstrating novel RCA-based biosensors with superior specificity, effi-ciency, sensitivity, and portability for the detection of food-borne microorganisms. In the meanwhile, the emerging RCA biosensing technology, as a future development prospect of RCA, is proposed as well. This review provides a comprehensive reference for the fields of biomolecular science, disease diagnosis, and health care.(c) 2021 Elsevier B.V. All rights reserved.