Viral and immune factors associated with successful treatment withdrawal in HBeAg-negative chronic hepatitis B patients

•Stopping NA is feasible in a high proportion of HBeAg-negative CHB patients.•Low baseline HBsAg titres identify patients who will achieve functional cure.•Reduced cccDNA activity is associated with HBsAg loss after NA discontinuation.•HBV-specific T cell functionality may influence patient outcome upon NA withdrawal.