H-1 NMR Metabonomic Investigations of N-Benzylhexadecanamide Induced Proliferation and Iestosterone Secretion of Mouse Testicular Leydig Cellst

A metabolomic mechanism was evaluated for inducing proliferation and testosterone secretion of mouse testicular Leydig cells by N-benzylhexadecanamide ( NBH) from maca based on 1H NMR metabolism approach. The cell proliferation rate of mouse testicular Leydig cell sbefore and after administration and the testosterone content in the cell culture medium was determined experimentally. Based on principal component analysis ( PCA) and orthogonal partial least squares discriminant analysis ( OPLS-DA), metabolite differences in mouse testicular Leydig cells before and after administration were studied, and metabolic pathway analysis was performed. The results showed that NBH could increase the proliferation rate and testosterone secretion of mouse testicular Leydig cells. After administration, the profile of the faulty liquid metabolic in mouse testicular Leydig cells was changed significantly, which identified 24distinct metabolites such as leucine, lysine, scylloinositol, valine and alanine. Metaboanalyst showed that distinct metabolites mainly involves 10 pathways including alanine, aspartate and glutamate metabolism, glycine, serine and threonine metabolism, glutathione metabolism, pyruvate metabolism, pantothenate and CoA biosynthesis, aminoacyl-tRNA biosynthesis, lysine degradation, Inositol phosphate metabolism, D-glutamine and D -glutamate metabolism as well as glycerophospholipid metabolism. The result indicated that NBH promotes proliferation and secretion of testosterone in mouse Leydig cells by organizing the relevant nodes of the above metabolic pathway.